Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1863970..1864624 Replicon chromosome
Accession NZ_CP070124
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K22_RS09035 Protein ID WP_000244781.1
Coordinates 1864217..1864624 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K22_RS09030 Protein ID WP_000354046.1
Coordinates 1863970..1864236 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS09010 (1860058) 1860058..1861491 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
I6K22_RS09015 (1861536) 1861536..1861847 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K22_RS09020 (1862011) 1862011..1862670 + 660 WP_000250274.1 hemolysin III family protein -
I6K22_RS09025 (1862747) 1862747..1863727 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
I6K22_RS09030 (1863970) 1863970..1864236 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K22_RS09035 (1864217) 1864217..1864624 + 408 WP_000244781.1 protein YgfX Toxin
I6K22_RS09040 (1864664) 1864664..1865185 - 522 WP_001055874.1 flavodoxin FldB -
I6K22_RS09045 (1865297) 1865297..1866193 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
I6K22_RS09050 (1866218) 1866218..1866928 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K22_RS09055 (1866934) 1866934..1868667 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T193231 WP_000244781.1 NZ_CP070124:1864217-1864624 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T193231 NZ_CP092788:132783-132932 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT193231 WP_000354046.1 NZ_CP070124:1863970-1864236 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT193231 NZ_CP092788:c132738-132679 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References