Detailed information of TA system TA193227

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 338167..339001
• Replicon: chromosome
• Accession: NZ_CP070124
• Organism: Escherichia coli strain FDAARGOS_1292

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: I6K22_RS01695
• Protein ID: WP_000854820.1
• Coordinates: 338624..339001 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: I6K22_RS01690
• Protein ID: WP_001285610.1
• Coordinates: 338167..338535 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K22_RS01650 (333888)	333888..334568	+	681	WP_001278649.1	WYL domain-containing protein	-
I6K22_RS01655 (334719)	334719..335396	+	678	WP_001097565.1	hypothetical protein	-
I6K22_RS01660 (335402)	335402..335635	+	234	WP_000883181.1	DUF905 family protein	-
I6K22_RS01665 (335725)	335725..336543	+	819	WP_001175155.1	DUF932 domain-containing protein	-
I6K22_RS01670 (336569)	336569..336709	-	141	WP_000997937.1	hypothetical protein	-
I6K22_RS01675 (336809)	336809..337288	+	480	WP_000706978.1	antirestriction protein	-
I6K22_RS01680 (337303)	337303..337779	+	477	WP_001186193.1	RadC family protein	-
I6K22_RS01685 (337866)	337866..338087	+	222	WP_000692347.1	DUF987 domain-containing protein	-
I6K22_RS01690 (338167)	338167..338535	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K22_RS01695 (338624)	338624..339001	+	378	WP_000854820.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
I6K22_RS01700 (338998)	338998..339195	+	198	Protein_341	DUF5983 family protein	-
I6K22_RS01705 (339223)	339223..339420	+	198	WP_085949158.1	DUF957 domain-containing protein	-
I6K22_RS01710 (339505)	339505..340065	+	561	Protein_343	DUF4942 domain-containing protein	-
I6K22_RS01715 (340899)	340899..342437	+	1539	WP_001619771.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX	278650..350250	71600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T193227 WP_000854820.1 NZ_CP070124:338624-339001 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T193227 NZ_CP092787:19053-19469 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT193227 WP_001285610.1 NZ_CP070124:338167-338535 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT193227 NZ_CP092787:18826-19056 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6