Detailed information of TA system TA193226

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 81722..82556
• Replicon: chromosome
• Accession: NZ_CP070124
• Organism: Escherichia coli strain FDAARGOS_1292

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: I6K22_RS00395
• Protein ID: WP_000854770.1
• Coordinates: 81722..82099 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K22_RS00400
• Protein ID: WP_001332042.1
• Coordinates: 82188..82556 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K22_RS00375 (80246)	80246..80422	-	177	Protein_76	helix-turn-helix domain-containing protein	-
I6K22_RS00380 (80789)	80789..80938	-	150	Protein_77	hypothetical protein	-
I6K22_RS00385 (81044)	81044..81220	-	177	WP_000839286.1	DUF957 domain-containing protein	-
I6K22_RS00390 (81237)	81237..81725	-	489	WP_000761690.1	DUF5983 family protein	-
I6K22_RS00395 (81722)	81722..82099	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
I6K22_RS00400 (82188)	82188..82556	-	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K22_RS00405 (82719)	82719..82940	-	222	WP_000692323.1	DUF987 domain-containing protein	-
I6K22_RS00410 (83003)	83003..83479	-	477	WP_001557203.1	RadC family protein	-
I6K22_RS00415 (83495)	83495..83959	-	465	WP_000855061.1	antirestriction protein	-
I6K22_RS00420 (84112)	84112..84300	-	189	WP_198545698.1	hypothetical protein	-
I6K22_RS00425 (84300)	84300..85118	-	819	WP_001234738.1	DUF932 domain-containing protein	-
I6K22_RS00430 (85236)	85236..85431	-	196	Protein_87	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	69800..106332	36532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T193226 WP_000854770.1 NZ_CP070124:c82099-81722 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T193226 NZ_CP092786:c5392175-5391792 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT193226 WP_001332042.1 NZ_CP070124:c82556-82188 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT193226 NZ_CP092786:c5392417-5392175 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9