Detailed information of TA system TA193224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 35640..36187
• Replicon: plasmid unnamed3
• Accession: NZ_CP070118
• Organism: Escherichia coli strain FDAARGOS_1298

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: I6K28_RS25940
• Protein ID: WP_001384452.1
• Coordinates: 35640..35918 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: I6K28_RS25945
• Protein ID: WP_000079928.1
• Coordinates: 35918..36187 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K28_RS25905 (30694)	30694..30885	-	192	WP_000175572.1	DUF2164 family protein	-
I6K28_RS25910 (31082)	31082..31183	-	102	Protein_49	methionyl-tRNA formyltransferase	-
I6K28_RS25915 (31198)	31198..31707	-	510	WP_000115001.1	peptide deformylase	-
I6K28_RS25920 (32235)	32235..32600	+	366	WP_016235422.1	hypothetical protein	-
I6K28_RS25925 (32600)	32600..33787	+	1188	Protein_52	IS91 family transposase	-
I6K28_RS25930 (34147)	34147..34629	-	483	WP_001311056.1	hypothetical protein	-
I6K28_RS25935 (34746)	34746..35594	-	849	WP_016235419.1	3'-5' exonuclease	-
I6K28_RS25940 (35640)	35640..35918	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
I6K28_RS25945 (35918)	35918..36187	-	270	WP_000079928.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
I6K28_RS25950 (37101)	37101..37958	-	858	WP_042018306.1	incFII family plasmid replication initiator RepA	-
I6K28_RS25955 (37951)	37951..38025	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6K28_RS25960 (38249)	38249..38509	-	261	WP_000083819.1	replication regulatory protein RepA	-
I6K28_RS25965 (38749)	38749..39339	-	591	WP_000766807.1	DUF2726 domain-containing protein	-
I6K28_RS25970 (39377)	39377..39586	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
I6K28_RS25975 (39632)	39632..40093	-	462	WP_001233838.1	thermonuclease family protein	-
I6K28_RS25980 (40339)	40339..40551	-	213	WP_151854166.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69978	69978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T193224 WP_001384452.1 NZ_CP070118:c35918-35640 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T193224 NZ_CP092786:c4910641-4910357 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT193224 WP_000079928.1 NZ_CP070118:c36187-35918 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT193224 NZ_CP092786:c4910873-4910631 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A5W2J4D0