Detailed information of TA system TA193222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 104830..105377
• Replicon: plasmid unnamed1
• Accession: NZ_CP070116
• Organism: Escherichia coli strain FDAARGOS_1298

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: I6K28_RS25290
• Protein ID: WP_001384452.1
• Coordinates: 105099..105377 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: I6K28_RS25285
• Protein ID: WP_000079941.1
• Coordinates: 104830..105099 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K28_RS25260 (100536)	100536..101339	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
I6K28_RS25265 (101339)	101339..102175	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
I6K28_RS25270 (102497)	102497..102751	+	255	WP_000083830.1	replication regulatory protein RepA	-
I6K28_RS26585 (102790)	102790..103026	-	237	WP_223228793.1	replication protein RepA	-
I6K28_RS26590 (103017)	103017..103064	+	48	WP_077822033.1	hypothetical protein	-
I6K28_RS25280 (103057)	103057..103914	+	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
I6K28_RS25285 (104830)	104830..105099	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
I6K28_RS25290 (105099)	105099..105377	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
I6K28_RS25295 (105423)	105423..106271	+	849	WP_001057989.1	3'-5' exonuclease	-
I6K28_RS25300 (106457)	106457..108337	+	1881	WP_001283335.1	colicin Ia central receptor-binding domain-containing protein	-
I6K28_RS25305 (108359)	108359..108694	-	336	WP_001080729.1	colicin 1A immunity protein	-
I6K28_RS25310 (108941)	108941..109345	+	405	WP_000839181.1	transposase	-
I6K28_RS25315 (109342)	109342..109689	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(B) / aph(3'')-Ib / aph(6)-Id	iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB	1..150643	150643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T193222 WP_001384452.1 NZ_CP070116:105099-105377 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T193222 NZ_CP092786:4194352-4194570 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT193222 WP_000079941.1 NZ_CP070116:104830-105099 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT193222 NZ_CP092786:4193951-4194325 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A629TXC5