Detailed information of TA system TA193220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4206227..4207815
• Replicon: chromosome
• Accession: NZ_CP070115
• Organism: Escherichia coli strain FDAARGOS_1298

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K28_RS20235
• Protein ID: WP_001125475.1
• Coordinates: 4206493..4207815 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K28_RS20230
• Protein ID: WP_001332145.1
• Coordinates: 4206227..4206493 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K28_RS20215 (4202595)	4202595..4204046	+	1452	WP_000854637.1	tagaturonate reductase	-
I6K28_RS20220 (4204243)	4204243..4205157	+	915	WP_001306069.1	bestrophin family protein	-
I6K28_RS20225 (4205161)	4205161..4205919	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K28_RS20230 (4206227)	4206227..4206493	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K28_RS20235 (4206493)	4206493..4207815	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K28_RS20240 (4208125)	4208125..4208307	-	183	Protein_3987	hypothetical protein	-
I6K28_RS20245 (4208441)	4208441..4209223	+	783	WP_001221834.1	SDR family oxidoreductase	-
I6K28_RS26545 (4209467)	4209467..4209574	-	108	Protein_3989	hypothetical protein	-
I6K28_RS20250 (4209999)	4209999..4210562	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
I6K28_RS20255 (4210924)	4210924..4211634	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T193220 WP_001125475.1 NZ_CP070115:4206493-4207815 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T193220 NZ_CP092786:2012420-2012522 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT193220 WP_001332145.1 NZ_CP070115:4206227-4206493 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT193220 NZ_CP092786:c2012529-2012384 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8