Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2595084..2595738 Replicon chromosome
Accession NZ_CP070115
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K28_RS12510 Protein ID WP_000244781.1
Coordinates 2595331..2595738 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K28_RS12505 Protein ID WP_000354046.1
Coordinates 2595084..2595350 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS12485 (2591172) 2591172..2592605 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
I6K28_RS12490 (2592650) 2592650..2592961 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K28_RS12495 (2593125) 2593125..2593784 + 660 WP_000250274.1 hemolysin III family protein -
I6K28_RS12500 (2593861) 2593861..2594841 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
I6K28_RS12505 (2595084) 2595084..2595350 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K28_RS12510 (2595331) 2595331..2595738 + 408 WP_000244781.1 protein YgfX Toxin
I6K28_RS12515 (2595778) 2595778..2596299 - 522 WP_001055874.1 flavodoxin FldB -
I6K28_RS12520 (2596411) 2596411..2597307 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
I6K28_RS12525 (2597332) 2597332..2598042 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K28_RS12530 (2598048) 2598048..2599781 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T193208 WP_000244781.1 NZ_CP070115:2595331-2595738 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T193208 NZ_CP092778:476842-477192 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT193208 WP_000354046.1 NZ_CP070115:2595084-2595350 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT193208 NZ_CP092778:476555-476836 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References