Detailed information of TA system TA193203

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1035414..1036248
• Replicon: chromosome
• Accession: NZ_CP070115
• Organism: Escherichia coli strain FDAARGOS_1298

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: I6K28_RS05030
• Protein ID: WP_000854820.1
• Coordinates: 1035871..1036248 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: I6K28_RS05025
• Protein ID: WP_001285610.1
• Coordinates: 1035414..1035782 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K28_RS04985 (1031135)	1031135..1031815	+	681	WP_113334534.1	WYL domain-containing protein	-
I6K28_RS04990 (1031966)	1031966..1032643	+	678	WP_001097565.1	hypothetical protein	-
I6K28_RS04995 (1032649)	1032649..1032882	+	234	WP_000883181.1	DUF905 family protein	-
I6K28_RS05000 (1032972)	1032972..1033790	+	819	WP_001175155.1	DUF932 domain-containing protein	-
I6K28_RS05005 (1033816)	1033816..1033956	-	141	WP_000997937.1	hypothetical protein	-
I6K28_RS05010 (1034056)	1034056..1034535	+	480	WP_113334503.1	antirestriction protein	-
I6K28_RS05015 (1034550)	1034550..1035026	+	477	WP_001186193.1	RadC family protein	-
I6K28_RS05020 (1035113)	1035113..1035334	+	222	WP_000692347.1	DUF987 domain-containing protein	-
I6K28_RS05025 (1035414)	1035414..1035782	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K28_RS05030 (1035871)	1035871..1036248	+	378	WP_000854820.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
I6K28_RS05035 (1036245)	1036245..1036442	+	198	Protein_995	DUF5983 family protein	-
I6K28_RS05040 (1036470)	1036470..1036667	+	198	WP_085949158.1	DUF957 domain-containing protein	-
I6K28_RS05045 (1036752)	1036752..1037312	+	561	Protein_997	DUF4942 domain-containing protein	-
I6K28_RS05050 (1038147)	1038147..1039685	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T193203 WP_000854820.1 NZ_CP070115:1035871-1036248 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T193203 NZ_CP092765:c5256946-5256563 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT193203 WP_001285610.1 NZ_CP070115:1035414-1035782 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT193203 NZ_CP092765:c5257188-5256946 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6