Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 211856..212474 Replicon chromosome
Accession NZ_CP070115
Organism Escherichia coli strain FDAARGOS_1298

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K28_RS01085 Protein ID WP_001291435.1
Coordinates 212256..212474 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K28_RS01080 Protein ID WP_000344800.1
Coordinates 211856..212230 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K28_RS01070 (206946) 206946..208139 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K28_RS01075 (208162) 208162..211311 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K28_RS01080 (211856) 211856..212230 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K28_RS01085 (212256) 212256..212474 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K28_RS01090 (212648) 212648..213199 + 552 WP_000102543.1 maltose O-acetyltransferase -
I6K28_RS01095 (213315) 213315..213785 + 471 WP_000136192.1 YlaC family protein -
I6K28_RS01100 (213949) 213949..215499 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K28_RS01105 (215541) 215541..215894 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K28_RS01115 (216273) 216273..216584 + 312 WP_000409908.1 MGMT family protein -
I6K28_RS01120 (216615) 216615..217187 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193199 WP_001291435.1 NZ_CP070115:212256-212474 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193199 NZ_CP092765:4059123-4059341 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193199 WP_000344800.1 NZ_CP070115:211856-212230 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193199 NZ_CP092765:4058722-4059096 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References