Detailed information of TA system TA193195

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4415899..4416553
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: I6K31_RS21220
• Protein ID: WP_000244781.1
• Coordinates: 4416146..4416553 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: I6K31_RS21215
• Protein ID: WP_016239318.1
• Coordinates: 4415899..4416165 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS21195 (4411987)	4411987..4413420	-	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-
I6K31_RS21200 (4413465)	4413465..4413776	+	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
I6K31_RS21205 (4413940)	4413940..4414599	+	660	WP_000250274.1	hemolysin III family protein	-
I6K31_RS21210 (4414676)	4414676..4415656	-	981	WP_000886078.1	tRNA-modifying protein YgfZ	-
I6K31_RS21215 (4415899)	4415899..4416165	+	267	WP_016239318.1	FAD assembly factor SdhE	Antitoxin
I6K31_RS21220 (4416146)	4416146..4416553	+	408	WP_000244781.1	protein YgfX	Toxin
I6K31_RS21225 (4416593)	4416593..4417114	-	522	WP_001055874.1	flavodoxin FldB	-
I6K31_RS21230 (4417226)	4417226..4418122	+	897	WP_000806987.1	site-specific tyrosine recombinase XerD	-
I6K31_RS21235 (4418147)	4418147..4418857	+	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
I6K31_RS21240 (4418863)	4418863..4420596	+	1734	WP_000813190.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T193195 WP_000244781.1 NZ_CP070111:4416146-4416553 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T193195 NZ_CP092765:741288-741773 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10573.20 Da
• Isoelectric Point: 5.1599

>AT193195 WP_016239318.1 NZ_CP070111:4415899-4416165 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDILSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT193195 NZ_CP092765:740998-741291 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S1PAM6