Detailed information of TA system TA193159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 574783..575261
• Replicon: chromosome
• Accession: NZ_CP070111
• Organism: Escherichia coli strain FDAARGOS_1301

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: D8A5B3
• Locus tag: I6K31_RS02465
• Protein ID: WP_000936799.1
• Coordinates: 574783..575070 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: D8A5B4
• Locus tag: I6K31_RS02470
• Protein ID: WP_000100899.1
• Coordinates: 575070..575261 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K31_RS02440 (572079)	572079..572300	-	222	WP_000560231.1	cell division protein FtsZ	-
I6K31_RS02445 (572346)	572346..573194	-	849	WP_000935606.1	Rha family phage regulatory protein	-
I6K31_RS02450 (573762)	573762..573983	+	222	WP_000202161.1	hypothetical protein	-
I6K31_RS02455 (573973)	573973..574347	-	375	WP_001553098.1	hypothetical protein	-
I6K31_RS02460 (574359)	574359..574511	-	153	WP_000379609.1	DUF1391 family protein	-
I6K31_RS02465 (574783)	574783..575070	-	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K31_RS02470 (575070)	575070..575261	-	192	WP_000100899.1	hypothetical protein	Antitoxin
I6K31_RS02475 (575289)	575289..575690	-	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
I6K31_RS02480 (575800)	575800..576072	+	273	WP_000887447.1	YdaS family helix-turn-helix protein	-
I6K31_RS02485 (576056)	576056..576481	+	426	WP_000693918.1	toxin YdaT family protein	-
I6K31_RS02490 (576553)	576553..577623	+	1071	WP_001262355.1	hypothetical protein	-
I6K31_RS02495 (577664)	577664..578089	+	426	WP_001151152.1	DUF977 family protein	-
I6K31_RS02500 (578264)	578264..578929	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
I6K31_RS02505 (579167)	579167..579322	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
I6K31_RS02510 (579490)	579490..579768	+	279	WP_024193993.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtP / ybtQ / ybtX / ybtS	559145..606437	47292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10929.85 Da
• Isoelectric Point: 10.1360

>T193159 WP_000936799.1 NZ_CP070111:c575070-574783 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENIPAARRLKIRIETSVLALSEHPYLYPPSDRVSGLREIVVHPNYIVLYRVATSSIE
IANIVHARRQFPFPI

Nucleotide

• Length: 288 bp

>T193159 NZ_CP092740:c19851-19510 [Leptospira interrogans]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGTTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7035.80 Da
• Isoelectric Point: 4.0270

>AT193159 WP_000100899.1 NZ_CP070111:c575261-575070 [Escherichia coli]
MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDEVMAEMENLIAQIAVTNRSE

Nucleotide

• Length: 192 bp

>AT193159 NZ_CP092740:c20081-19845 [Leptospira interrogans]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: D8A5B3

Antitoxin

• AlphaFold DB: A0A161RC07