Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2071265..2071890 Replicon chromosome
Accession NZ_CP070105
Organism Klebsiella pneumoniae strain FDAARGOS_1314

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K44_RS10215 Protein ID WP_002882817.1
Coordinates 2071507..2071890 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K44_RS10210 Protein ID WP_004150355.1
Coordinates 2071265..2071507 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K44_RS10185 2067255..2067854 + 600 WP_004151865.1 glucose-1-phosphatase -
I6K44_RS10190 2067848..2068708 + 861 WP_004146232.1 virulence factor BrkB family protein -
I6K44_RS10195 2068705..2069142 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K44_RS10200 2069187..2070128 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K44_RS10205 2070143..2071060 - 918 WP_023313778.1 alpha/beta hydrolase -
I6K44_RS10210 2071265..2071507 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K44_RS10215 2071507..2071890 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K44_RS10220 2072064..2072993 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K44_RS10225 2072990..2073625 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K44_RS10230 2073622..2074524 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T193149 WP_002882817.1 NZ_CP070105:2071507-2071890 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T193149 NZ_CP092733:1028513-1029811 [Comamonas thiooxydans]
ATGACGCATGAACTCGAAGTCTGGCTGTTTGATGGGCGCGTCGGCAGTCTGTCGCTGGTGCATGGGCGGCTGAGCTTTGC
CTATGCGACCGACTGGTTGGCGCTGCCGCAGGCCACGGCTTTATCGCAGTCGCTGCCGCTGCAGGCTAAACCTTTTGACG
ATCATCAGACCCGGCCGTTCTTCGCCGGTCTGCTGCCCGAGGGGCAGATGCGGCGCCTGATTGCCCAGCAGTTTCAGGTG
TCGGGCCAAAACGATTTTGCGCTGCTGGACCATATTGGCGGCGAATGCGCGGGGGCCGTGACCTTTCTGGAGCCGGGGCA
GGCCTTGCCGCAGACTAACGGGGCTGGGGCGACCGGGGCACCGGGTGATGTGGAGTGGCTCAGCGATCAAAAGCTGCTCG
CCTTGCTGGACGAGCTGCCGCGTCGCCCCATGCTGGCGGGAGATGATGGGCTGCGGCTATCGCTGGCAGGGGCGCAGGAC
AAGTTGCCCGTGGTGGTGGACGAGAGCATTCCGGGCGCAGTGCGTATCGGTCTGCCGCGTAACGGCGCGCCCAGCTCGCA
TATCTTGAAGCCCGCCATTCATGCTGTGGAGGACAGTGTGGCCAATGAAGGCTTTTGCATGCTGCTGGCCCAGGTCATGG
GGCTGCGGCCTGCCAATGCCCGAATCCAGAGCGTGCTGGGCCGCTCGTTTTTGCTGGTGAAGCGCTATGACCGGGAGCTG
AATGCTCAAGATCTGCACCAGCCGCAGTTGCAACGTCTGCATCAGGAAGACTTTTGCCAGGCGCTGGGCGTGGTGCCCGA
GCTGAAGTACCAGAACGAGGGCGGGCCGGGCCTGGCGCAATGCTTTGCCCTGCTGCGCAGCGTGACCCGGCCCAGCGCGC
CTCAGGTGCTGCGGCTGCTGGATGGGGCGATCTTCAATGCCTTGATTGGCAACCATGATGCCCATGGCAAGAATTTCTCG
CTGCTTTATGGGGGAAGGGCGCCCGTGCTGGCACCGTTCTACGACCTGCTGGCTACAGCGATTTACCCCCAGCTGACGCC
CAAGATGGCAATGAAGATTGGCAGCAAATACAAGTTCAGCGAGCTGGAGGCCAGGCACTGGGAGCAGTTTGCGGAAGAAG
CAGGCCTTGCCAAGGCGGCGACGCGCAAGCGTTTGCAGCAGCTGGCGAACGAACTACCCGCTGCCGCGCGCAAGCTGCAG
GCCGCGCCACCGCATGGTTTTGCGGGCAATGCCGTGGTGACGCAGATCGTGCAGCTGATAGAGCAGCGCTGCGCCTTGAC
GCTGAGGCGCTTGGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT193149 WP_004150355.1 NZ_CP070105:2071265-2071507 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT193149 NZ_CP092733:1028275-1028523 [Comamonas thiooxydans]
ATGTCCACCATCCAAACAACCGAGCAACTGGGCGCGGCCTTGCGCACCGCCCGTAAACAACTGGGGCTGACCCAGCCCCA
GCTTGCCTTGGCCGCAGGCGTGGGCACGCGTTTTATTGTGGATCTCGAGGCTGGCAAGCCCACGGTGCGGCTGGAGCATG
TGCTGCGGGTGATCGATGCGCTGGGCGGGGAGTTGCAGTTGGGCGGCTTGCCGAATACAGCTTCTGAGGGCGCAGACCAT
GACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References