Detailed information of TA system TA193126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3653429..3654067
• Replicon: chromosome
• Accession: NZ_CP070103
• Organism: Escherichia coli strain FDAARGOS_1277

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I6K07_RS17355
• Protein ID: WP_000813794.1
• Coordinates: 3653429..3653605 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K07_RS17360
• Protein ID: WP_001270286.1
• Coordinates: 3653651..3654067 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K07_RS17335 (3649048)	3649048..3650223	-	1176	WP_001236265.1	BenE family transporter YdcO	-
I6K07_RS17340 (3650315)	3650315..3650851	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
I6K07_RS17345 (3650924)	3650924..3652885	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K07_RS17350 (3652977)	3652977..3653207	-	231	WP_000494244.1	YncJ family protein	-
I6K07_RS17355 (3653429)	3653429..3653605	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K07_RS17360 (3653651)	3653651..3654067	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K07_RS17365 (3654146)	3654146..3655552	+	1407	WP_085004608.1	PLP-dependent aminotransferase family protein	-
I6K07_RS17370 (3655797)	3655797..3656941	+	1145	Protein_3416	ABC transporter substrate-binding protein	-
I6K07_RS17375 (3656959)	3656959..3657972	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
I6K07_RS17380 (3657973)	3657973..3658914	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T193126 WP_000813794.1 NZ_CP070103:3653429-3653605 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T193126 NZ_CP092713:c1036377-1036060 [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGTCACAATTTACGCTTTATAAAAACAAAGATAAAAGCTCAACTAAAACGTACCCTTACTTTGTAGATGTTCAAAGTGA
TTTACTCGATAACCTAAATACTCGATTAGTCATTCCATTAACGCCAATTGAACTACTTGATAAAAAAGCCCCAAGTCACC
TTTGTCCTACAATTCATATTGATGAAGGTGACTTTGTCATGCTAACTCAACAAATGACAAGCGTTCCGGTTAAGATCCTA
ACCGATCCAGTTAATGAATTATCGACTTTCAGAAATGAAATTATTGCTGCAATTGATTTTTTAATCACTGGCATTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT193126 WP_001270286.1 NZ_CP070103:3653651-3654067 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT193126 NZ_CP092713:c1036623-1036378 [Aliivibrio fischeri ATCC 7744 = JCM 18803 = DSM 507]
ATGAGAAGTCAATACAATACACAAGCGATAAAAAAAGCAACCAATCTAACATTAAACAGCGATCTACTCGCTGAAGCAAA
ACGATTAAAAATAAACCTTTCAGCAACAATGGAAAAAGCATTATCACAAGAAGTAAGTAAACTAAAAAGACAAGAGTGGT
TAGAACAAAACTCAGAAGCTATTGATGCTTGCAACGAACTAACCGAGAAACATGGTCTTTTTTCTGATTCATACCGAGTA
TTTTAA

Structures

Toxin

• PDB: 6HPB