Detailed information of TA system TA193117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3070024..3070729
• Replicon: chromosome
• Accession: NZ_CP070103
• Organism: Escherichia coli strain FDAARGOS_1277

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K07_RS14580
• Protein ID: WP_000539521.1
• Coordinates: 3070343..3070729 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K07_RS14575
• Protein ID: WP_001280945.1
• Coordinates: 3070024..3070353 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K07_RS14560 (3065181)	3065181..3066092	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
I6K07_RS14565 (3066270)	3066270..3068618	+	2349	WP_085004572.1	EAL domain-containing protein	-
I6K07_RS14570 (3068626)	3068626..3069954	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
I6K07_RS14575 (3070024)	3070024..3070353	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K07_RS14580 (3070343)	3070343..3070729	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K07_RS14585 (3070955)	3070955..3072280	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K07_RS14590 (3072493)	3072493..3072876	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K07_RS14595 (3072987)	3072987..3074102	+	1116	WP_085004566.1	aldose sugar dehydrogenase YliI	-
I6K07_RS14600 (3074099)	3074099..3074725	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T193117 WP_000539521.1 NZ_CP070103:c3070729-3070343 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T193117 NZ_CP092702:c4579787-4579446 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT193117 WP_001280945.1 NZ_CP070103:c3070353-3070024 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT193117 NZ_CP092702:4579782-4579858 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A829GCT6