Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2444746..2445533 Replicon chromosome
Accession NZ_CP070103
Organism Escherichia coli strain FDAARGOS_1277

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag I6K07_RS11465 Protein ID WP_001194695.1
Coordinates 2444746..2445123 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag I6K07_RS11470 Protein ID WP_000066236.1
Coordinates 2445174..2445533 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K07_RS11425 (2439985) 2439985..2440740 - 756 WP_001052717.1 hydroxyacylglutathione hydrolase -
I6K07_RS11430 (2440774) 2440774..2441496 + 723 WP_001297210.1 class I SAM-dependent methyltransferase -
I6K07_RS11435 (2441493) 2441493..2441960 - 468 WP_000917883.1 ribonuclease HI -
I6K07_RS11440 (2442025) 2442025..2442756 + 732 WP_001300756.1 DNA polymerase III subunit epsilon -
I6K07_RS11450 (2443098) 2443098..2443942 - 845 Protein_2237 DUF4942 domain-containing protein -
I6K07_RS11455 (2444023) 2444023..2444226 - 204 WP_000413747.1 DUF957 domain-containing protein -
I6K07_RS11460 (2444258) 2444258..2444749 - 492 WP_000777682.1 DUF5983 family protein -
I6K07_RS11465 (2444746) 2444746..2445123 - 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
I6K07_RS11470 (2445174) 2445174..2445533 - 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
I6K07_RS11475 (2445557) 2445557..2445778 - 222 WP_000691981.1 DUF987 domain-containing protein -
I6K07_RS11480 (2445799) 2445799..2446278 - 480 WP_000437750.1 DNA repair protein RadC -
I6K07_RS11485 (2446290) 2446290..2446760 - 471 WP_000131762.1 antirestriction protein -
I6K07_RS11490 (2447028) 2447028..2447846 - 819 WP_001234406.1 DUF932 domain-containing protein -
I6K07_RS11495 (2447966) 2447966..2448199 - 234 WP_001114682.1 DUF905 domain-containing protein -
I6K07_RS11500 (2448276) 2448276..2448728 - 453 WP_001061894.1 IrmA family protein -
I6K07_RS11505 (2448788) 2448788..2449330 - 543 WP_001104018.1 DUF4339 domain-containing protein -
I6K07_RS11510 (2449393) 2449393..2449845 - 453 WP_000734138.1 hypothetical protein -
I6K07_RS11515 (2449842) 2449842..2450294 - 453 WP_001020418.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T193113 WP_001194695.1 NZ_CP070103:c2445123-2444746 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T193113 NZ_CP092702:c3700034-3699927 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT193113 WP_000066236.1 NZ_CP070103:c2445533-2445174 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT193113 NZ_CP092702:3700083-3700149 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References