Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 580845..581499 Replicon chromosome
Accession NZ_CP070103
Organism Escherichia coli strain FDAARGOS_1277

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6K07_RS02745 Protein ID WP_000244777.1
Coordinates 580845..581252 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K07_RS02750 Protein ID WP_000354046.1
Coordinates 581233..581499 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K07_RS02725 (576802) 576802..578535 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
I6K07_RS02730 (578541) 578541..579251 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K07_RS02735 (579276) 579276..580172 - 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
I6K07_RS02740 (580284) 580284..580805 + 522 WP_001055874.1 flavodoxin FldB -
I6K07_RS02745 (580845) 580845..581252 - 408 WP_000244777.1 protein YgfX Toxin
I6K07_RS02750 (581233) 581233..581499 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K07_RS02755 (581742) 581742..582722 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6K07_RS02760 (582918) 582918..583577 - 660 WP_000250274.1 hemolysin III family protein -
I6K07_RS02765 (583741) 583741..584052 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I6K07_RS02770 (584097) 584097..585530 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
I6K07_RS02775 (585587) 585587..586330 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T193107 WP_000244777.1 NZ_CP070103:c581252-580845 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T193107 NZ_CP092702:2777432-2777761 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT193107 WP_000354046.1 NZ_CP070103:c581499-581233 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT193107 NZ_CP092702:2777094-2777411 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References