Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4722153..4722650 Replicon chromosome
Accession NZ_CP070098
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6L00_RS23110 Protein ID WP_001173343.1
Coordinates 4722477..4722650 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6L00_RS23105 Protein ID WP_000398619.1
Coordinates 4722153..4722449 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS23085 (4718882) 4718882..4719601 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6L00_RS23090 (4719598) 4719598..4721229 - 1632 WP_001216477.1 sensor histidine kinase -
I6L00_RS23095 (4721410) 4721410..4721640 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6L00_RS23100 (4721652) 4721652..4721924 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6L00_RS23105 (4722153) 4722153..4722449 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6L00_RS23110 (4722477) 4722477..4722650 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6L00_RS23115 (4722769) 4722769..4724286 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6L00_RS23120 (4724523) 4724523..4725980 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T193097 WP_001173343.1 NZ_CP070098:4722477-4722650 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T193097 NZ_CP092702:c1270345-1270238 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT193097 WP_000398619.1 NZ_CP070098:4722153-4722449 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT193097 NZ_CP092702:1270392-1270458 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References