Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3086672..3087255 Replicon chromosome
Accession NZ_CP070098
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag I6L00_RS15500 Protein ID WP_033550297.1
Coordinates 3086672..3087007 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6L00_RS15505 Protein ID WP_000581937.1
Coordinates 3087007..3087255 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS15480 (3081908) 3081908..3082366 - 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
I6L00_RS15485 (3082559) 3082559..3083857 - 1299 WP_000036723.1 phosphopyruvate hydratase -
I6L00_RS15490 (3083944) 3083944..3085581 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I6L00_RS15495 (3085809) 3085809..3086600 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
I6L00_RS15500 (3086672) 3086672..3087007 - 336 WP_033550297.1 endoribonuclease MazF Toxin
I6L00_RS15505 (3087007) 3087007..3087255 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6L00_RS15510 (3087333) 3087333..3089567 - 2235 WP_000226798.1 GTP diphosphokinase -
I6L00_RS15515 (3089615) 3089615..3090916 - 1302 WP_001521172.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3081908..3082366 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12112.07 Da        Isoelectric Point: 8.2618

>T193092 WP_033550297.1 NZ_CP070098:c3087007-3086672 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALAEQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T193092 NZ_CP092702:252301-252699 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT193092 WP_000581937.1 NZ_CP070098:c3087255-3087007 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT193092 NZ_CP092702:252005-252298 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References