Detailed information of TA system TA193080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1514201..1514839
• Replicon: chromosome
• Accession: NZ_CP070098
• Organism: Escherichia coli strain FDAARGOS_1370

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: I6L00_RS07830
• Protein ID: WP_001306887.1
• Coordinates: 1514201..1514377 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6L00_RS07835
• Protein ID: WP_001270286.1
• Coordinates: 1514423..1514839 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L00_RS07805 (1509257)	1509257..1509715	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-
I6L00_RS07810 (1509823)	1509823..1510995	-	1173	WP_001523225.1	BenE family transporter YdcO	-
I6L00_RS07815 (1511087)	1511087..1511623	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
I6L00_RS07820 (1511696)	1511696..1513657	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6L00_RS07825 (1513749)	1513749..1513979	-	231	WP_000494241.1	YncJ family protein	-
I6L00_RS07830 (1514201)	1514201..1514377	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6L00_RS07835 (1514423)	1514423..1514839	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6L00_RS07840 (1514918)	1514918..1516324	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
I6L00_RS07845 (1516569)	1516569..1517714	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
I6L00_RS07850 (1517732)	1517732..1518745	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
I6L00_RS07855 (1518746)	1518746..1519687	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1509257..1509715	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T193080 WP_001306887.1 NZ_CP070098:1514201-1514377 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T193080 NZ_CP070098:1514201-1514377 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT193080 WP_001270286.1 NZ_CP070098:1514423-1514839 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT193080 NZ_CP070098:1514423-1514839 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76