Detailed information of TA system TA193072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 816198..816903
• Replicon: chromosome
• Accession: NZ_CP070098
• Organism: Escherichia coli strain FDAARGOS_1370

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6L00_RS04345
• Protein ID: WP_000539521.1
• Coordinates: 816517..816903 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6L00_RS04340
• Protein ID: WP_001280945.1
• Coordinates: 816198..816527 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L00_RS04325 (811355)	811355..812266	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
I6L00_RS04330 (812444)	812444..814792	+	2349	WP_021523051.1	EAL domain-containing protein	-
I6L00_RS04335 (814800)	814800..816128	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
I6L00_RS04340 (816198)	816198..816527	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6L00_RS04345 (816517)	816517..816903	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L00_RS04350 (817129)	817129..818454	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6L00_RS04355 (818667)	818667..819050	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6L00_RS04360 (819161)	819161..820276	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I6L00_RS04365 (820273)	820273..820899	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T193072 WP_000539521.1 NZ_CP070098:c816903-816517 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T193072 NZ_CP092682:c1445422-1445144 [Vibrio campbellii]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAATATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTGATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTATCTTACTGGGTTGAGGGCGATATCATCCGTGTTATG
CGTGTACTTCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT193072 WP_001280945.1 NZ_CP070098:c816527-816198 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT193072 NZ_CP092682:c1445703-1445419 [Vibrio campbellii]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TTTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6