Detailed information of TA system TA193070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 185910..187599
• Replicon: chromosome
• Accession: NZ_CP070098
• Organism: Escherichia coli strain FDAARGOS_1370

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: I6L00_RS01535
• Protein ID: WP_001521912.1
• Coordinates: 186679..187599 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: I6L00_RS01530
• Protein ID: WP_001521911.1
• Coordinates: 185910..186692 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L00_RS01505 (181219)	181219..181377	-	159	Protein_160	integrase	-
I6L00_RS01510 (181469)	181469..181571	-	103	Protein_161	helix-turn-helix domain-containing protein	-
I6L00_RS01515 (181568)	181568..182038	-	471	WP_000201185.1	hypothetical protein	-
I6L00_RS01525 (182385)	182385..185594	-	3210	WP_001521910.1	RNA-directed DNA polymerase	-
I6L00_RS01530 (185910)	185910..186692	+	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I6L00_RS01535 (186679)	186679..187599	+	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I6L00_RS01540 (188033)	188033..188917	-	885	WP_001119037.1	LysR family transcriptional regulator	-
I6L00_RS01545 (189182)	189182..190240	+	1059	WP_122452191.1	alpha/beta hydrolase	-
I6L00_RS01550 (190382)	190382..191509	+	1128	WP_001521913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T193070 WP_001521912.1 NZ_CP070098:186679-187599 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T193070 NZ_CP092681:c1035844-1035446 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT193070 WP_001521911.1 NZ_CP070098:185910-186692 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT193070 NZ_CP092681:c1036074-1035844 [Leptospira interrogans]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTTATCCAAAAGGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A829L165