193066

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 30591..31116 Replicon plasmid unnamed1
Accession NZ_CP070097
Organism Escherichia coli strain FDAARGOS_1370

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag I6L00_RS00220 Protein ID WP_001159868.1
Coordinates 30811..31116 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6L00_RS00215 Protein ID WP_000813634.1
Coordinates 30591..30809 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L00_RS00200 (25747) 25747..26646 + 900 WP_000963206.1 nucleotide-binding protein -
I6L00_RS00205 (26696) 26696..28921 - 2226 WP_000698737.1 P-loop NTPase fold protein -
I6L00_RS00210 (28923) 28923..30011 - 1089 WP_000952217.1 transcriptional repressor PifC -
I6L00_RS00215 (30591) 30591..30809 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6L00_RS00220 (30811) 30811..31116 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
I6L00_RS00225 (31117) 31117..31923 + 807 WP_000016982.1 site-specific integrase -
I6L00_RS00230 (32697) 32697..33452 + 756 WP_000852146.1 replication initiation protein RepE -
I6L00_RS00235 (34040) 34040..35206 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / mph(A) / sul1 / aadA5 senB 1..104896 104896


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T193066 WP_001159868.1 NZ_CP070097:30811-31116 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T193066 NZ_CP092676:3164799-3165197 [Leptospira interrogans]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT193066 WP_000813634.1 NZ_CP070097:30591-30809 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT193066 NZ_CP092676:3164569-3164799 [Leptospira interrogans]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGGGAGAGAACAACCGAAATCTGATAAAAGAGAAAAACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References