Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 25014..25750 Replicon plasmid unnamed1
Accession NZ_CP070093
Organism Klebsiella pneumoniae strain FDAARGOS_1316

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag I6K46_RS25625 Protein ID WP_003026803.1
Coordinates 25014..25496 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K46_RS25630 Protein ID WP_003026799.1
Coordinates 25484..25750 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K46_RS25610 21470..22816 - 1347 WP_020314316.1 ISNCY family transposase -
I6K46_RS25615 23007..23411 - 405 WP_009484309.1 helix-turn-helix domain-containing protein -
I6K46_RS25620 23460..24806 - 1347 WP_077249638.1 ISNCY family transposase -
I6K46_RS25625 25014..25496 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
I6K46_RS25630 25484..25750 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K46_RS25635 25926..26180 - 255 WP_004152108.1 hypothetical protein -
I6K46_RS25640 26256..26513 - 258 WP_004152107.1 hypothetical protein -
I6K46_RS25645 26562..26765 - 204 WP_004152106.1 hemolysin expression modulator Hha -
I6K46_RS25650 26799..27167 - 369 WP_004152105.1 hypothetical protein -
I6K46_RS25655 27211..27705 - 495 WP_004152104.1 DNA-binding protein -
I6K46_RS25660 27736..28311 - 576 WP_004152103.1 hypothetical protein -
I6K46_RS25665 28299..28568 - 270 WP_004152102.1 hypothetical protein -
I6K46_RS25670 28926..29276 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
I6K46_RS25675 29326..29688 + 363 WP_204752961.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / qnrB19 / aph(6)-Id - 1..210510 210510


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T193065 WP_003026803.1 NZ_CP070093:c25496-25014 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T193065 NZ_CP092676:c2747080-2746739 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTAAAGTTGAAATTGGACTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT193065 WP_003026799.1 NZ_CP070093:c25750-25484 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT193065 NZ_CP092676:c2747315-2747067 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References