Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1366135..1366760 Replicon chromosome
Accession NZ_CP070092
Organism Klebsiella pneumoniae strain FDAARGOS_1316

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K46_RS06640 Protein ID WP_002882817.1
Coordinates 1366377..1366760 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K46_RS06635 Protein ID WP_004150355.1
Coordinates 1366135..1366377 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K46_RS06610 1362126..1362725 + 600 WP_004151865.1 glucose-1-phosphatase -
I6K46_RS06615 1362719..1363579 + 861 WP_004146232.1 virulence factor BrkB family protein -
I6K46_RS06620 1363576..1364013 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K46_RS06625 1364058..1364999 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K46_RS06630 1365013..1365930 - 918 WP_204752955.1 alpha/beta hydrolase fold domain-containing protein -
I6K46_RS06635 1366135..1366377 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K46_RS06640 1366377..1366760 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K46_RS06645 1366934..1367863 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K46_RS06650 1367860..1368495 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K46_RS06655 1368492..1369394 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T193051 WP_002882817.1 NZ_CP070092:1366377-1366760 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T193051 NZ_CP092656:1195377-1195862 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT193051 WP_004150355.1 NZ_CP070092:1366135-1366377 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT193051 NZ_CP092656:1195087-1195380 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References