Detailed information of TA system TA193048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 82101..82837
• Replicon: plasmid unnamed1
• Accession: NZ_CP070089
• Organism: Klebsiella pneumoniae strain FDAARGOS_1306

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I6K36_RS26540
• Protein ID: WP_003026803.1
• Coordinates: 82355..82837 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I6K36_RS26535
• Protein ID: WP_003026799.1
• Coordinates: 82101..82367 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K36_RS26490	78163..78525	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I6K36_RS26495	78575..78925	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I6K36_RS26500	79283..79552	+	270	WP_019706036.1	hypothetical protein	-
I6K36_RS26505	79540..80115	+	576	WP_004152103.1	hypothetical protein	-
I6K36_RS26510	80146..80640	+	495	WP_004152104.1	DNA-binding protein	-
I6K36_RS26515	80684..81052	+	369	WP_004152105.1	hypothetical protein	-
I6K36_RS26520	81086..81289	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I6K36_RS26525	81338..81595	+	258	WP_004152107.1	hypothetical protein	-
I6K36_RS26530	81671..81925	+	255	WP_004152108.1	hypothetical protein	-
I6K36_RS26535	82101..82367	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I6K36_RS26540	82355..82837	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I6K36_RS26545	82996..84250	-	1255	Protein_99	IS3 family transposase	-
I6K36_RS26550	84338..85300	-	963	WP_004152113.1	M48 family metalloprotease	-
I6K36_RS26555	85287..86036	-	750	WP_009483782.1	phosphate-starvation-inducible PsiE family protein	-
I6K36_RS26560	86274..86471	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207010	207010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T193048 WP_003026803.1 NZ_CP070089:82355-82837 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T193048 NZ_CP092656:c378152-377769 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT193048 WP_003026799.1 NZ_CP070089:82101-82367 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT193048 NZ_CP092656:c378394-378152 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66