Detailed information of TA system TA193040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2982605..2983415
• Replicon: chromosome
• Accession: NZ_CP070088
• Organism: Klebsiella pneumoniae strain FDAARGOS_1306

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I6K36_RS14480
• Protein ID: WP_083565061.1
• Coordinates: 2982605..2983138 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: I6K36_RS14485
• Protein ID: WP_002887278.1
• Coordinates: 2983149..2983415 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K36_RS14475	2981436..2982557	+	1122	WP_040188022.1	cupin domain-containing protein	-
I6K36_RS14480	2982605..2983138	-	534	WP_083565061.1	GNAT family N-acetyltransferase	Toxin
I6K36_RS14485	2983149..2983415	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
I6K36_RS14490	2983518..2984951	-	1434	WP_186983936.1	Cu(+)/Ag(+) sensor histidine kinase	-
I6K36_RS14495	2984941..2985624	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
I6K36_RS14500	2985796..2987181	+	1386	WP_048999682.1	efflux transporter outer membrane subunit	-
I6K36_RS14505	2987199..2987543	+	345	WP_204746220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19856.74 Da
• Isoelectric Point: 5.2612

>T193040 WP_083565061.1 NZ_CP070088:c2983138-2982605 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCCEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T193040 NZ_CP092652:1959681-1960205 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT193040 WP_002887278.1 NZ_CP070088:c2983415-2983149 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT193040 NZ_CP092652:1959406-1959684 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1