Detailed information of TA system TA193027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 214138..214728
• Replicon: chromosome
• Accession: NZ_CP070088
• Organism: Klebsiella pneumoniae strain FDAARGOS_1306

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: I6K36_RS00935
• Protein ID: WP_023341911.1
• Coordinates: 214396..214728 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: I6K36_RS00930
• Protein ID: WP_000288812.1
• Coordinates: 214138..214395 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K36_RS00910	210473..212221	+	1749	WP_100127257.1	hypothetical protein	-
I6K36_RS00915	212275..212856	+	582	WP_099184326.1	hypothetical protein	-
I6K36_RS00920	213139..213600	+	462	WP_004213450.1	hypothetical protein	-
I6K36_RS00925	213597..213803	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
I6K36_RS00930	214138..214395	+	258	WP_000288812.1	antitoxin	Antitoxin
I6K36_RS00935	214396..214728	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6K36_RS00945	215050..216486	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
I6K36_RS00955	216852..218306	-	1455	WP_004148975.1	AMP nucleosidase	-
I6K36_RS00960	218436..218681	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T193027 WP_023341911.1 NZ_CP070088:214396-214728 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T193027 NZ_CP092648:2401876-2403198 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT193027 WP_000288812.1 NZ_CP070088:214138-214395 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT193027 NZ_CP092648:2401610-2401876 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACATGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0