Detailed information of TA system TA193008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1590..2064
• Replicon: plasmid unnamed5
• Accession: NZ_CP070085
• Organism: Klebsiella pneumoniae strain FDAARGOS_1328

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: I6K58_RS01420
• Protein ID: WP_004197762.1
• Coordinates: 1798..2064 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: I6K58_RS01415
• Protein ID: WP_000879771.1
• Coordinates: 1590..1814 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K58_RS01405	195..704	+	510	WP_032433972.1	MbeB family mobilization protein	-
I6K58_RS01410	711..923	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
I6K58_RS01415	1590..1814	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
I6K58_RS01420	1798..2064	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K58_RS01425	2134..2484	-	351	WP_004197756.1	hypothetical protein	-
I6K58_RS01430	2550..3095	-	546	WP_032433973.1	hypothetical protein	-
I6K58_RS01435	3121..3502	-	382	Protein_6	hypothetical protein	-
I6K58_RS01440	4592..4768	+	177	WP_165764108.1	hypothetical protein	-
I6K58_RS01445	4758..5102	+	345	WP_004197764.1	MobC family plasmid mobilization relaxosome protein	-
I6K58_RS01450	5041..5559	+	519	Protein_9	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5585	5585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T193008 WP_004197762.1 NZ_CP070085:1798-2064 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T193008 NZ_CP092647:c1532576-1532169 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT193008 WP_000879771.1 NZ_CP070085:1590-1814 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT193008 NZ_CP092647:c1532823-1532557 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5