Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20984..21627 Replicon plasmid unnamed3
Accession NZ_CP070084
Organism Klebsiella pneumoniae strain FDAARGOS_1328

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag I6K58_RS01295 Protein ID WP_000754566.1
Coordinates 21211..21627 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag I6K58_RS01290 Protein ID WP_001261276.1
Coordinates 20984..21214 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K58_RS01275 18493..19269 - 777 WP_000015958.1 site-specific integrase -
I6K58_RS01280 19266..20009 - 744 WP_000129823.1 hypothetical protein -
I6K58_RS01285 20060..20410 - 351 WP_000493378.1 hypothetical protein -
I6K58_RS01290 20984..21214 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6K58_RS01295 21211..21627 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K58_RS01300 21831..22687 + 857 Protein_24 IS3-like element ISEc15 family transposase -
I6K58_RS01305 22969..24045 + 1077 WP_000227969.1 IS110 family transposase -
I6K58_RS01310 24686..24769 + 84 Protein_26 SOS response-associated peptidase -
I6K58_RS01315 24932..25789 - 858 Protein_27 Tn3 family transposase -
I6K58_RS01320 25875..26627 + 753 WP_000602738.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA2 / sul3 / aph(4)-Ia / aac(3)-IVa - 1..42567 42567
- inside IScluster/Tn aadA2 / sul3 / aph(4)-Ia / aac(3)-IVa - 332..30701 30369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T193007 WP_000754566.1 NZ_CP070084:21211-21627 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T193007 NZ_CP092647:c1389724-1389389 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT193007 WP_001261276.1 NZ_CP070084:20984-21214 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT193007 NZ_CP092647:c1389972-1389724 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References