Detailed information of TA system TA193006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 19881..20617
• Replicon: plasmid unnamed1
• Accession: NZ_CP070083
• Organism: Klebsiella pneumoniae strain FDAARGOS_1328

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I6K58_RS00200
• Protein ID: WP_003026803.1
• Coordinates: 19881..20363 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I6K58_RS00205
• Protein ID: WP_003026799.1
• Coordinates: 20351..20617 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K58_RS00185	15518..16480	+	963	WP_004152113.1	M48 family metalloprotease	-
I6K58_RS00190	16568..17822	+	1255	Protein_20	IS3 family transposase	-
I6K58_RS00195	18323..19669	-	1347	WP_020314316.1	ISNCY family transposase	-
I6K58_RS00200	19881..20363	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I6K58_RS00205	20351..20617	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I6K58_RS00210	20793..21047	-	255	WP_004152108.1	hypothetical protein	-
I6K58_RS00215	21123..21380	-	258	WP_004152107.1	hypothetical protein	-
I6K58_RS00220	21429..21632	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
I6K58_RS00225	21666..22034	-	369	WP_004152105.1	hypothetical protein	-
I6K58_RS00230	22078..22572	-	495	WP_004152104.1	DNA-binding protein	-
I6K58_RS00235	22603..23178	-	576	WP_004152103.1	hypothetical protein	-
I6K58_RS00240	23166..23435	-	270	WP_004152102.1	hypothetical protein	-
I6K58_RS00245	23793..24143	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I6K58_RS00250	24193..24555	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..208838	208838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T193006 WP_003026803.1 NZ_CP070083:c20363-19881 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T193006 NZ_CP092647:c1062012-1061614 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT193006 WP_003026799.1 NZ_CP070083:c20617-20351 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT193006 NZ_CP092647:c1062239-1062012 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66