Detailed information of TA system TA192991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3233456..3234039
• Replicon: chromosome
• Accession: NZ_CP070077
• Organism: Escherichia coli strain FDAARGOS_1371

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6L01_RS15175
• Protein ID: WP_033550297.1
• Coordinates: 3233704..3234039 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: I6L01_RS15170
• Protein ID: WP_000581937.1
• Coordinates: 3233456..3233704 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L01_RS15160 (3229795)	3229795..3231096	+	1302	WP_001521172.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
I6L01_RS15165 (3231144)	3231144..3233378	+	2235	WP_000226798.1	GTP diphosphokinase	-
I6L01_RS15170 (3233456)	3233456..3233704	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I6L01_RS15175 (3233704)	3233704..3234039	+	336	WP_033550297.1	endoribonuclease MazF	Toxin
I6L01_RS15180 (3234111)	3234111..3234902	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
I6L01_RS15185 (3235130)	3235130..3236767	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
I6L01_RS15190 (3236854)	3236854..3238152	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
I6L01_RS15195 (3238345)	3238345..3238803	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3238345..3238803	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12112.07 Da
• Isoelectric Point: 8.2618

>T192991 WP_033550297.1 NZ_CP070077:3233704-3234039 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALAEQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T192991 NZ_CP092639:c2380918-2380742 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT192991 WP_000581937.1 NZ_CP070077:3233456-3233704 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT192991 NZ_CP092639:c2380696-2380280 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LMB4