192986

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1654822..1655319 Replicon chromosome
Accession NZ_CP070077
Organism Escherichia coli strain FDAARGOS_1371

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6L01_RS07860 Protein ID WP_001173343.1
Coordinates 1654822..1654995 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6L01_RS07865 Protein ID WP_000398619.1
Coordinates 1655023..1655319 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L01_RS07850 (1651492) 1651492..1652949 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
I6L01_RS07855 (1653186) 1653186..1654703 + 1518 WP_000003806.1 lysine--tRNA ligase -
I6L01_RS07860 (1654822) 1654822..1654995 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6L01_RS07865 (1655023) 1655023..1655319 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6L01_RS07870 (1655548) 1655548..1655820 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6L01_RS07875 (1655832) 1655832..1656062 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6L01_RS07880 (1656243) 1656243..1657874 + 1632 WP_001216477.1 sensor histidine kinase -
I6L01_RS07885 (1657871) 1657871..1658590 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1609477..1655319 45842


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192986 WP_001173343.1 NZ_CP070077:c1654995-1654822 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192986 NZ_CP092639:1775068-1775319 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT192986 WP_000398619.1 NZ_CP070077:c1655319-1655023 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192986 NZ_CP092639:1775316-1775570 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References