Detailed information of TA system TA192977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 87734..88518
• Replicon: chromosome
• Accession: NZ_CP070077
• Organism: Escherichia coli strain FDAARGOS_1371

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6L01_RS00550
• Protein ID: WP_000613626.1
• Coordinates: 88024..88518 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6L01_RS00545
• Protein ID: WP_001110447.1
• Coordinates: 87734..88027 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L01_RS00535 (82884)	82884..83843	-	960	WP_023153797.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
I6L01_RS00540 (84416)	84416..87601	+	3186	WP_001522867.1	ribonuclease E	-
I6L01_RS00545 (87734)	87734..88027	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6L01_RS00550 (88024)	88024..88518	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6L01_RS00555 (88613)	88613..89566	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6L01_RS00560 (89578)	89578..91221	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I6L01_RS00565 (91287)	91287..92228	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6L01_RS00570 (92228)	92228..93325	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192977 WP_000613626.1 NZ_CP070077:88024-88518 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192977 NZ_CP092635:c5256927-5256616 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGATGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTTCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCATACCCACGAGTTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192977 WP_001110447.1 NZ_CP070077:87734-88027 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192977 NZ_CP092635:c5256615-5256325 [Enterobacter cloacae]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGACGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAATGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6