Detailed information of TA system TA192961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4163354..4163985
• Replicon: chromosome
• Accession: NZ_CP070071
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: I6K17_RS20940
• Protein ID: WP_001260301.1
• Coordinates: 4163354..4163629 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: I6K17_RS20945
• Protein ID: WP_000593557.1
• Coordinates: 4163626..4163985 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS20925 (4158358)	4158358..4159425	+	1068	WP_000361470.1	HlyD family secretion protein	-
I6K17_RS20930 (4159422)	4159422..4162157	+	2736	WP_204608607.1	ribosome-associated ATPase/putative transporter RbbA	-
I6K17_RS20935 (4162157)	4162157..4163281	+	1125	WP_001318088.1	ABC transporter permease	-
I6K17_RS20940 (4163354)	4163354..4163629	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
I6K17_RS20945 (4163626)	4163626..4163985	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I6K17_RS20950 (4164105)	4164105..4164506	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
I6K17_RS20955 (4164512)	4164512..4165318	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
I6K17_RS20960 (4165315)	4165315..4166079	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
I6K17_RS20965 (4166079)	4166079..4166912	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
I6K17_RS20970 (4166909)	4166909..4167853	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T192961 WP_001260301.1 NZ_CP070071:4163354-4163629 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T192961 NZ_CP092634:c152319-152038 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT192961 WP_000593557.1 NZ_CP070071:4163626-4163985 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT192961 NZ_CP092634:c152543-152316 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1