Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3342857..3343354 Replicon chromosome
Accession NZ_CP070071
Organism Escherichia coli strain FDAARGOS_1287

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K17_RS17185 Protein ID WP_001173343.1
Coordinates 3342857..3343030 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag I6K17_RS17190 Protein ID WP_001317561.1
Coordinates 3343058..3343354 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K17_RS17175 (3339527) 3339527..3340984 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
I6K17_RS17180 (3341221) 3341221..3342738 + 1518 WP_000003805.1 lysine--tRNA ligase -
I6K17_RS17185 (3342857) 3342857..3343030 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K17_RS17190 (3343058) 3343058..3343354 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K17_RS17195 (3343581) 3343581..3343853 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K17_RS17200 (3343865) 3343865..3344095 - 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
I6K17_RS17205 (3344275) 3344275..3345906 + 1632 WP_001216477.1 sensor histidine kinase -
I6K17_RS17210 (3345903) 3345903..3346622 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192952 WP_001173343.1 NZ_CP070071:c3343030-3342857 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192952 NZ_CP092632:c1181557-1181177 [Klebsiella variicola subsp. variicola]
ATGCAAACTTTACCGTTACCCGAGCCACGGGCGGCTGAGCCCTGCCCGTCGCCTGTGCAGGTCTGGCAGTTGCTGCTCAC
ACATCTGCTGGATAAACACTATGGCCTCACGCTCAAAGACACACCCTTTGGCGACGACAGCGTGATTCAGGCGCACATCA
ACGCCGGTATCTCCCTGTGCGATGCTGTCAATTTCATTGTGGAAAGGTATGAACTGGTGCGCACTGACCTTAGCCGTTTC
AGCCTCCCCGAACGCTCTTCACTGATTGGTGCCATCGACATACTGCGTGCCCGTCGTGCAACCGGACTGATAGTAAGCAG
CGGCTACAGCACCATAACCCGCATCACAACGGGCCGGTATCGGAAGGACAAAACACAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT192952 WP_001317561.1 NZ_CP070071:c3343354-3343058 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192952 NZ_CP092632:c1181915-1181589 [Klebsiella variicola subsp. variicola]
ATGCATTCTCAACAATGGGGGCTAACGTGTAGTAAAACGCCGTGCTTCGGTGCCAGGCTGGTGCAGGAAGGCAACCGACT
GCACTTTCTGGCTGACCGGGCAGGATTTACCGGCGATTTCAGCGACGATGAAGCACTGCGCCTTGACCAGGCTTTTCCAC
TGATAATGAAACAGCTGGAACAGATGCTTCCAGCTGGTGAGCTGAATCCTCGCCATCAGCAACGCGTTACCCTGAATCAC
ATTGGGCTGACCTGCGAAGCTGATACGCTTGGCTCACAAGGTTATGTCTACATCTCGATTTATCCCTCTTCGACTGTAAC
CGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References