Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2459963..2460581 Replicon chromosome
Accession NZ_CP070071
Organism Escherichia coli strain FDAARGOS_1287

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K17_RS12925 Protein ID WP_001291435.1
Coordinates 2460363..2460581 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K17_RS12920 Protein ID WP_000344800.1
Coordinates 2459963..2460337 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K17_RS12910 (2455052) 2455052..2456245 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K17_RS12915 (2456268) 2456268..2459417 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K17_RS12920 (2459963) 2459963..2460337 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K17_RS12925 (2460363) 2460363..2460581 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K17_RS12930 (2460753) 2460753..2461304 + 552 WP_000102564.1 maltose O-acetyltransferase -
I6K17_RS12935 (2461420) 2461420..2461890 + 471 WP_000136192.1 YlaC family protein -
I6K17_RS12940 (2462054) 2462054..2463604 + 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K17_RS12945 (2463646) 2463646..2463999 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K17_RS12955 (2464378) 2464378..2464689 + 312 WP_000409911.1 MGMT family protein -
I6K17_RS12960 (2464720) 2464720..2465292 - 573 WP_000779821.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192949 WP_001291435.1 NZ_CP070071:2460363-2460581 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192949 NZ_CP092631:c2238286-2238110 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTTCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192949 WP_000344800.1 NZ_CP070071:2459963-2460337 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192949 NZ_CP092631:2238227-2238327 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGAAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References