Detailed information of TA system TA192946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1797203..1797987
• Replicon: chromosome
• Accession: NZ_CP070071
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6K17_RS09615
• Protein ID: WP_000613624.1
• Coordinates: 1797493..1797987 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: I6K17_RS09610
• Protein ID: WP_001110446.1
• Coordinates: 1797203..1797496 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS09600 (1792352)	1792352..1793311	-	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
I6K17_RS09605 (1793884)	1793884..1797069	+	3186	WP_000827395.1	ribonuclease E	-
I6K17_RS09610 (1797203)	1797203..1797496	+	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
I6K17_RS09615 (1797493)	1797493..1797987	+	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
I6K17_RS09620 (1798082)	1798082..1799035	-	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
I6K17_RS09625 (1799047)	1799047..1800690	-	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
I6K17_RS09630 (1800756)	1800756..1801697	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K17_RS09635 (1801697)	1801697..1802794	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T192946 WP_000613624.1 NZ_CP070071:1797493-1797987 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192946 NZ_CP092631:c1330042-1329296 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT192946 WP_001110446.1 NZ_CP070071:1797203-1797496 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192946 NZ_CP092631:c1329296-1329126 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775