Detailed information of TA system TA192945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1490363..1490999
• Replicon: chromosome
• Accession: NZ_CP070071
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: I6K17_RS08095
• Protein ID: WP_000813796.1
• Coordinates: 1490823..1490999 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K17_RS08090
• Protein ID: WP_076838470.1
• Coordinates: 1490363..1490779 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS08070 (1485515)	1485515..1486456	-	942	WP_001251307.1	ABC transporter permease	-
I6K17_RS08075 (1486457)	1486457..1487470	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
I6K17_RS08080 (1487488)	1487488..1488633	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
I6K17_RS08085 (1488878)	1488878..1490284	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
I6K17_RS08090 (1490363)	1490363..1490779	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K17_RS08095 (1490823)	1490823..1490999	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K17_RS08100 (1491221)	1491221..1491451	+	231	WP_000494243.1	YncJ family protein	-
I6K17_RS08105 (1491543)	1491543..1493504	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K17_RS08110 (1493577)	1493577..1494113	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
I6K17_RS08115 (1494205)	1494205..1495377	+	1173	WP_001236216.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T192945 WP_000813796.1 NZ_CP070071:c1490999-1490823 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T192945 NZ_CP092631:522905-523255 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT192945 WP_076838470.1 NZ_CP070071:c1490779-1490363 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192945 NZ_CP092631:522619-522900 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3