Detailed information of TA system TA192929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 94177..94724
• Replicon: plasmid unnamed1
• Accession: NZ_CP070070
• Organism: Escherichia coli strain FDAARGOS_1287

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: I6K17_RS00525
• Protein ID: WP_001384452.1
• Coordinates: 94177..94455 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: I6K17_RS00530
• Protein ID: WP_032181987.1
• Coordinates: 94455..94724 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K17_RS00495 (90031)	90031..90450	-	420	Protein_97	protein YagA	-
I6K17_RS00500 (90804)	90804..91406	+	603	WP_045133169.1	ProQ/FINO family protein	-
I6K17_RS00505 (91462)	91462..91956	+	495	WP_001350009.1	hypothetical protein	-
I6K17_RS00510 (91956)	91956..92222	+	267	WP_000627831.1	hypothetical protein	-
I6K17_RS00515 (92699)	92699..93148	-	450	WP_053871401.1	hypothetical protein	-
I6K17_RS00520 (93289)	93289..94137	-	849	WP_001057996.1	3'-5' exonuclease	-
I6K17_RS00525 (94177)	94177..94455	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
I6K17_RS00530 (94455)	94455..94724	-	270	WP_032181987.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
I6K17_RS00535 (94779)	94779..94937	-	159	WP_000841008.1	hypothetical protein	-
I6K17_RS00540 (95633)	95633..96664	-	1032	WP_044706701.1	plasmid replication initiator RepA	-
I6K17_RS00545 (97670)	97670..97900	+	231	WP_228772074.1	ash family protein	-
I6K17_RS00550 (97818)	97818..98105	+	288	WP_000074849.1	conjugal transfer protein TraA	-
I6K17_RS00555 (98547)	98547..99080	+	534	WP_001306770.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..112030	112030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T192929 WP_001384452.1 NZ_CP070070:c94455-94177 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T192929 NZ_CP092627:3625494-3625757 [Salmonella enterica subsp. enterica serovar Thompson]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGGCGAGCGTTGTTGTGAAGATCTCCGAGCGGGGTTTGATCCTGATTG
CAGAAACGGATGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTA
AATAATGTGGTGAACGGGAATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.47 Da
• Isoelectric Point: 6.7251

>AT192929 WP_032181987.1 NZ_CP070070:c94724-94455 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT192929 NZ_CP092627:c3625747-3625337 [Salmonella enterica subsp. enterica serovar Thompson]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCATCCGTTTCTGCAATCAGGATCAAACCCCGCTCGGAGATCTTCACAACAACGCTCGCCCCGGTCTCAAATCCCGCTT
CTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGGCG
CCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAG
CGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCCGG
GGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A829CL36