Detailed information of TA system TA192912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2559332..2560920
• Replicon: chromosome
• Accession: NZ_CP070066
• Organism: Escherichia coli strain FDAARGOS_1296

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K26_RS12265
• Protein ID: WP_001125475.1
• Coordinates: 2559598..2560920 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K26_RS12260
• Protein ID: WP_001332145.1
• Coordinates: 2559332..2559598 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K26_RS12245 (2555700)	2555700..2557151	+	1452	WP_000854637.1	tagaturonate reductase	-
I6K26_RS12250 (2557348)	2557348..2558262	+	915	WP_001306069.1	bestrophin family protein	-
I6K26_RS12255 (2558266)	2558266..2559024	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K26_RS12260 (2559332)	2559332..2559598	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K26_RS12265 (2559598)	2559598..2560920	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K26_RS12270 (2561230)	2561230..2561412	-	183	Protein_2420	hypothetical protein	-
I6K26_RS12275 (2561546)	2561546..2562328	+	783	WP_001221834.1	SDR family oxidoreductase	-
I6K26_RS24405 (2562572)	2562572..2562679	-	108	Protein_2422	hypothetical protein	-
I6K26_RS12280 (2563104)	2563104..2563667	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
I6K26_RS12285 (2564029)	2564029..2564739	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T192912 WP_001125475.1 NZ_CP070066:2559598-2560920 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192912 NZ_CP092589:1930561-1930663 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT192912 WP_001332145.1 NZ_CP070066:2559332-2559598 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192912 NZ_CP092589:c1930670-1930525 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8