Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2004477..2005308 Replicon chromosome
Accession NZ_CP070066
Organism Escherichia coli strain FDAARGOS_1296

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K26_RS09645 Protein ID WP_000854814.1
Coordinates 2004477..2004851 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K26_RS09650 Protein ID WP_001285586.1
Coordinates 2004940..2005308 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K26_RS09605 (1999874) 1999874..2001039 + 1166 Protein_1893 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K26_RS09610 (2001158) 2001158..2001631 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K26_RS09615 (2001829) 2001829..2002887 + 1059 WP_001200890.1 FUSC family protein -
I6K26_RS09620 (2003059) 2003059..2003388 + 330 WP_000450409.1 DUF496 family protein -
I6K26_RS09625 (2003489) 2003489..2003623 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K26_RS09630 (2003725) 2003725..2003871 + 147 Protein_1898 transposase domain-containing protein -
I6K26_RS09635 (2004160) 2004160..2004240 - 81 Protein_1899 hypothetical protein -
I6K26_RS09640 (2004286) 2004286..2004480 - 195 WP_000988601.1 DUF5983 family protein -
I6K26_RS09645 (2004477) 2004477..2004851 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K26_RS09650 (2004940) 2004940..2005308 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K26_RS09655 (2005382) 2005382..2005603 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K26_RS09660 (2005672) 2005672..2006148 - 477 WP_001298074.1 RadC family protein -
I6K26_RS09665 (2006163) 2006163..2006636 - 474 WP_000855071.1 antirestriction protein -
I6K26_RS09670 (2006899) 2006899..2007720 - 822 WP_001234570.1 DUF932 domain-containing protein -
I6K26_RS09675 (2007941) 2007941..2008351 - 411 WP_000846704.1 hypothetical protein -
I6K26_RS09680 (2008367) 2008367..2009044 - 678 WP_001362823.1 hypothetical protein -
I6K26_RS09685 (2009180) 2009180..2010250 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192903 WP_000854814.1 NZ_CP070066:c2004851-2004477 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192903 NZ_CP092588:340848-341147 [Vibrio gazogenes]
ATGAAACCATTCCAACTGACCAATAGGGCGAAGTCTGATTTAAAAGACATCGCTCTATTTACCTATCACCGGTGGGGGCG
CGAACAGCGAAATGCTTACGTAAAACAATTCGACGAGACCTTTTGGCTATTAGCTGAAAACCCTGATATCGGTAAAAACT
GTGATGAAATCAGAAATGGCTACAAAAAGTTTCCTCAAGGCAGCCATGTTATTTTTTATCAACAAATCAGTAGCCAAAAT
ATTCAAATCATCAGAATCTTACATAAAAGTATGGATGTAAATCCTATATTTGGCGCATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT192903 WP_001285586.1 NZ_CP070066:c2005308-2004940 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192903 NZ_CP092588:340609-340851 [Vibrio gazogenes]
ATGGCTAAAAATACCAGCATTACTTTGGGTGAACACTTTGATAGCTTTATTGCTAACCAGATTCAAAGCGGACGCTATGG
TTCTGCGAGCGAAGTTATCCGCTCTGCTCTACGCTTACTTGAAACGCAAGAAACGAAAATGAACACCTTACGACAACTAC
TTGTTGAAGGTGAAGAGAGTGGTTTGGCTGACTATGACCTCAATTCGTTTATCAATGAACTCGATAGCGAAGAGAAAAAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References