Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4201400..4202054 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag I6L02_RS20010 Protein ID WP_000244765.1
Coordinates 4201400..4201807 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6L02_RS20015 Protein ID WP_000354046.1
Coordinates 4201788..4202054 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS19990 (4197357) 4197357..4199090 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
I6L02_RS19995 (4199096) 4199096..4199806 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6L02_RS20000 (4199831) 4199831..4200727 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6L02_RS20005 (4200839) 4200839..4201360 + 522 WP_001055874.1 flavodoxin FldB -
I6L02_RS20010 (4201400) 4201400..4201807 - 408 WP_000244765.1 protein YgfX Toxin
I6L02_RS20015 (4201788) 4201788..4202054 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6L02_RS20020 (4202297) 4202297..4203277 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
I6L02_RS20025 (4203354) 4203354..4204013 - 660 WP_000250274.1 hemolysin III family protein -
I6L02_RS20030 (4204177) 4204177..4204488 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6L02_RS20035 (4204533) 4204533..4205966 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T192897 WP_000244765.1 NZ_CP070063:c4201807-4201400 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192897 NZ_CP092581:c2076867-2076505 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192897 WP_000354046.1 NZ_CP070063:c4202054-4201788 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192897 NZ_CP092581:c2077034-2076864 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References