Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3247818..3248408 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag I6L02_RS15590 Protein ID WP_000373724.1
Coordinates 3247818..3248150 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag I6L02_RS15595 Protein ID WP_000288812.1
Coordinates 3248151..3248408 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS15565 (3243281) 3243281..3244543 + 1263 WP_000055690.1 integrase arm-type DNA-binding domain-containing protein -
I6L02_RS15570 (3244682) 3244682..3245140 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
I6L02_RS15575 (3246092) 3246092..3246970 - 879 WP_000511773.1 integrase domain-containing protein -
I6L02_RS15585 (3247349) 3247349..3247771 + 423 WP_001164104.1 hypothetical protein -
I6L02_RS15590 (3247818) 3247818..3248150 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6L02_RS15595 (3248151) 3248151..3248408 - 258 WP_000288812.1 antitoxin Antitoxin
I6L02_RS15600 (3248506) 3248506..3248697 + 192 WP_077561361.1 hypothetical protein -
I6L02_RS15605 (3248756) 3248756..3248962 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
I6L02_RS15610 (3248959) 3248959..3249426 - 468 WP_000201267.1 hypothetical protein -
I6L02_RS15615 (3249659) 3249659..3250795 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3233068..3267854 34786
- inside IScluster/Tn - - 3244682..3250795 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T192892 WP_000373724.1 NZ_CP070063:c3248150-3247818 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T192892 NZ_CP092578:c50786-50445 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT192892 WP_000288812.1 NZ_CP070063:c3248408-3248151 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT192892 NZ_CP092578:c51016-50786 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAACAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References