Detailed information of TA system TA192883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2775162..2776750
• Replicon: chromosome
• Accession: NZ_CP070063
• Organism: Escherichia coli strain FDAARGOS_1372

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I6L02_RS13200
• Protein ID: WP_001125428.1
• Coordinates: 2775162..2776484 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: I6L02_RS13205
• Protein ID: WP_001304368.1
• Coordinates: 2776484..2776750 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L02_RS13170 (2770281)	2770281..2771345	-	1065	WP_000859696.1	alpha/beta fold hydrolase	-
I6L02_RS13175 (2771503)	2771503..2772264	-	762	WP_001066620.1	carboxymuconolactone decarboxylase family protein	-
I6L02_RS13180 (2772242)	2772242..2772607	-	366	WP_000274389.1	cupin domain-containing protein	-
I6L02_RS23520 (2772785)	2772785..2773027	-	243	Protein_2601	two-component sensor histidine kinase	-
I6L02_RS13185 (2773109)	2773109..2773456	-	348	WP_001337832.1	hypothetical protein	-
I6L02_RS13190 (2773458)	2773458..2773952	-	495	WP_001337831.1	AEC family transporter	-
I6L02_RS13195 (2774399)	2774399..2774857	+	459	Protein_2604	acetylxylan esterase	-
I6L02_RS13200 (2775162)	2775162..2776484	-	1323	WP_001125428.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6L02_RS13205 (2776484)	2776484..2776750	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6L02_RS13210 (2777058)	2777058..2777816	+	759	WP_001286543.1	trans-aconitate 2-methyltransferase	-
I6L02_RS13215 (2777820)	2777820..2778734	-	915	WP_001313828.1	bestrophin family protein	-
I6L02_RS13220 (2778931)	2778931..2780382	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2755297..2776768	21471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.98 Da
• Isoelectric Point: 8.1297

>T192883 WP_001125428.1 NZ_CP070063:c2776484-2775162 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
FRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192883 NZ_CP092576:676358-676474 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACGCTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT192883 WP_001304368.1 NZ_CP070063:c2776750-2776484 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT192883 NZ_CP092576:c676590-676385 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACCAGTTATAAGAACTGGTGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGCGTTGCAAAACCAATCATCAGTCC

Structures

Antitoxin

• AlphaFold DB: V0VAH2