Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1699435..1700053 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6L02_RS07830 Protein ID WP_001291435.1
Coordinates 1699435..1699653 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6L02_RS07835 Protein ID WP_000344800.1
Coordinates 1699679..1700053 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS07795 (1694723) 1694723..1695295 + 573 WP_000779831.1 YbaY family lipoprotein -
I6L02_RS07800 (1695326) 1695326..1695637 - 312 WP_000409908.1 MGMT family protein -
I6L02_RS07810 (1696016) 1696016..1696369 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6L02_RS07815 (1696411) 1696411..1697961 - 1551 WP_001441899.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6L02_RS07820 (1698125) 1698125..1698595 - 471 WP_000136192.1 YlaC family protein -
I6L02_RS07825 (1698711) 1698711..1699262 - 552 WP_000102539.1 maltose O-acetyltransferase -
I6L02_RS07830 (1699435) 1699435..1699653 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6L02_RS07835 (1699679) 1699679..1700053 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6L02_RS07840 (1700598) 1700598..1703747 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6L02_RS07845 (1703770) 1703770..1704963 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192876 WP_001291435.1 NZ_CP070063:c1699653-1699435 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192876 NZ_CP092574:c1668657-1668295 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192876 WP_000344800.1 NZ_CP070063:c1700053-1699679 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192876 NZ_CP092574:c1668914-1668651 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References