Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1258777..1259327 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6L02_RS05790 Protein ID WP_001160966.1
Coordinates 1259013..1259327 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6L02_RS05785 Protein ID WP_000125566.1
Coordinates 1258777..1259010 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS05760 (1253850) 1253850..1254137 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6L02_RS05765 (1254196) 1254196..1255527 + 1332 WP_001183207.1 MFS transporter -
I6L02_RS05770 (1255635) 1255635..1256165 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6L02_RS05775 (1256158) 1256158..1258020 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
I6L02_RS05780 (1258212) 1258212..1258691 + 480 WP_204673701.1 type 3 dihydrofolate reductase -
I6L02_RS05785 (1258777) 1258777..1259010 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6L02_RS05790 (1259013) 1259013..1259327 + 315 WP_001160966.1 CcdB family protein Toxin
I6L02_RS05795 (1259324) 1259324..1260172 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6L02_RS05800 (1260179) 1260179..1260556 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6L02_RS05805 (1260559) 1260559..1261380 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6L02_RS05810 (1261377) 1261377..1262366 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6L02_RS05815 (1262366) 1262366..1263652 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T192874 WP_001160966.1 NZ_CP070063:1259013-1259327 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T192874 NZ_CP092574:676217-676333 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACGCTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT192874 WP_000125566.1 NZ_CP070063:1258777-1259010 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT192874 NZ_CP092574:c676449-676244 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACCAGTTATAAGAACTGGTGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGCGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References