Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 926888..927385 Replicon chromosome
Accession NZ_CP070063
Organism Escherichia coli strain FDAARGOS_1372

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6L02_RS04230 Protein ID WP_001173343.1
Coordinates 927212..927385 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6L02_RS04225 Protein ID WP_001312331.1
Coordinates 926888..927184 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L02_RS04205 (923617) 923617..924336 - 720 WP_000611282.1 two-component system response regulator DcuR -
I6L02_RS04210 (924333) 924333..925964 - 1632 WP_001216466.1 sensor histidine kinase -
I6L02_RS04215 (926145) 926145..926375 + 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
I6L02_RS04220 (926387) 926387..926659 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6L02_RS04225 (926888) 926888..927184 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6L02_RS04230 (927212) 927212..927385 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6L02_RS04235 (927504) 927504..929021 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6L02_RS04240 (929258) 929258..930715 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192870 WP_001173343.1 NZ_CP070063:927212-927385 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192870 NZ_CP092569:195374-195640 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGACAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAGAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT192870 WP_001312331.1 NZ_CP070063:926888-927184 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192870 NZ_CP092569:195111-195374 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References