Detailed information of TA system TA192847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3514303..3514915
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: I6K20_RS17140
• Protein ID: WP_000833473.1
• Coordinates: 3514730..3514915 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: I6K20_RS17135
• Protein ID: WP_000499744.1
• Coordinates: 3514303..3514713 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS17120 (3509815)	3509815..3510630	-	816	WP_000891825.1	AraC family transcriptional regulator	-
I6K20_RS17125 (3510856)	3510856..3512256	+	1401	WP_000204802.1	MFS transporter	-
I6K20_RS17130 (3512267)	3512267..3514231	+	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
I6K20_RS17135 (3514303)	3514303..3514713	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I6K20_RS17140 (3514730)	3514730..3514915	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
I6K20_RS17145 (3515193)	3515193..3516731	-	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
I6K20_RS17150 (3516932)	3516932..3518083	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T192847 WP_000833473.1 NZ_CP070048:c3514915-3514730 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T192847 NZ_CP092561:c2442009-2441743 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT192847 WP_000499744.1 NZ_CP070048:c3514713-3514303 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT192847 NZ_CP092561:c2442260-2442009 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9