Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2658772..2659426 Replicon chromosome
Accession NZ_CP070048
Organism Escherichia coli strain FDAARGOS_1290

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag I6K20_RS12945 Protein ID WP_000244769.1
Coordinates 2658772..2659179 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K20_RS12950 Protein ID WP_000354046.1
Coordinates 2659160..2659426 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K20_RS12925 (2654729) 2654729..2656462 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
I6K20_RS12930 (2656468) 2656468..2657178 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K20_RS12935 (2657203) 2657203..2658099 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
I6K20_RS12940 (2658211) 2658211..2658732 + 522 WP_001055874.1 flavodoxin FldB -
I6K20_RS12945 (2658772) 2658772..2659179 - 408 WP_000244769.1 protein YgfX Toxin
I6K20_RS12950 (2659160) 2659160..2659426 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K20_RS12955 (2659669) 2659669..2660649 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
I6K20_RS12960 (2660726) 2660726..2661385 - 660 WP_000250274.1 hemolysin III family protein -
I6K20_RS12965 (2661549) 2661549..2661860 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
I6K20_RS12970 (2661905) 2661905..2663338 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T192842 WP_000244769.1 NZ_CP070048:c2659179-2658772 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192842 NZ_CP092561:c851576-851388 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192842 WP_000354046.1 NZ_CP070048:c2659426-2659160 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192842 NZ_CP092561:c851865-851605 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References