Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1698548..1699379 Replicon chromosome
Accession NZ_CP070048
Organism Escherichia coli strain FDAARGOS_1290

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3K2V2C4
Locus tag I6K20_RS08565 Protein ID WP_001510972.1
Coordinates 1699005..1699379 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag I6K20_RS08560 Protein ID WP_001285585.1
Coordinates 1698548..1698916 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K20_RS08530 (1695452) 1695452..1695907 + 456 WP_000581504.1 IrmA family protein -
I6K20_RS08535 (1695986) 1695986..1696219 + 234 WP_001119724.1 DUF905 family protein -
I6K20_RS08540 (1696319) 1696319..1697137 + 819 WP_001234629.1 DUF932 domain-containing protein -
I6K20_RS08545 (1697219) 1697219..1697698 + 480 WP_000860076.1 antirestriction protein -
I6K20_RS08550 (1697714) 1697714..1698190 + 477 WP_001186773.1 RadC family protein -
I6K20_RS08555 (1698253) 1698253..1698474 + 222 WP_000692323.1 DUF987 domain-containing protein -
I6K20_RS08560 (1698548) 1698548..1698916 + 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K20_RS08565 (1699005) 1699005..1699379 + 375 WP_001510972.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K20_RS08570 (1699376) 1699376..1699570 + 195 WP_000988600.1 DUF5983 family protein -
I6K20_RS08575 (1699616) 1699616..1699696 + 81 Protein_1691 hypothetical protein -
I6K20_RS25205 (1699985) 1699985..1700065 - 81 WP_023441679.1 hypothetical protein -
I6K20_RS25210 (1700044) 1700044..1700367 + 324 WP_225343796.1 EutP/PduV family microcompartment system protein -
I6K20_RS08590 (1700468) 1700468..1700797 - 330 WP_000450409.1 DUF496 family protein -
I6K20_RS08595 (1700969) 1700969..1702027 - 1059 WP_001200891.1 FUSC family protein -
I6K20_RS08600 (1702225) 1702225..1702698 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
I6K20_RS08605 (1702817) 1702817..1703983 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13924.98 Da        Isoelectric Point: 7.8522

>T192840 WP_001510972.1 NZ_CP070048:1699005-1699379 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192840 NZ_CP092558:c2495658-2495392 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT192840 WP_001285585.1 NZ_CP070048:1698548-1698916 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192840 NZ_CP092558:c2495915-2495658 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3K2V2C4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References